Factors that may modulate collagen production include cancer, hypoxia, genetic disorders, inflammation, and aging (6-12). It is the main component of connective tissues and plays a critical role in the inflammatory process of rheumatic diseases (3-5). The collagen family of proteins is the most abundant protein family in mammals, comprising approximately 30% of total protein mass (2,3). The purpose of the ECM is to provide a homeostatic environment for cells to reside in, and although the demands for cell homeostasis differ between tissues and organs, the major constituent of ECMs is collagen (1). The extracellular matrix (ECM) is a highly dynamic and complex three-dimensional structure composed of a macromolecule network including collagen, glycosaminoglycans (GAGs), elastin, fibronectin, and several other non-cellular structures. Sirius Red/Fast Green Collagen Staining.Dextran Sulfate Sodium (DSS) for Colitis.MTT Cell Proliferation and Viability Assay.Mouse Anti-Crude Peanut Extract (CPE) (New!).CAIA - Collagen Antibody Induced Arthritis.Physical activity-related injuries in walkers and runners in the aerobics center longitudinal study. Chondroprotective effect of intraarticular injections of interleukin-1 receptor antagonist in experimental osteoarthritis. Clin Sci (Lond) 1997 92(4):415–422.Ĭaron JP, Fernandes JC, Martel-Pelletier J, Tardif G, Mineau F, Geng C, Pelletier JP. Mild endotoxaemia and the inflammatory response induced by a marathon race. 1997 499(Pt 3):833–841.Ĭamus G, Poortmans J, Nys M, Deby-Dupont G, Duchateau J, Deby C, Lamy M. Exercise-induced increase in serum interleukin-6 in humans is related to muscle damage. doi: 10.1074/jbc.24.īruunsgaard H, Galbo H, Halkjaer-Kristensen J, Johansen TL, MacLean DA, Pedersen BK. Nitric oxide is a physiological substrate for mammalian peroxidases. Furthermore, Coll2-1NO2 was not correlated with the total and active MPO indicating that Coll2-1 nitration did not result of a systemic oxidative phenomenon but reflects local changes.īiomarkers Cartilage Endurance exercise Type II collagen.Ību-Soud HM, Hazen SL. The serum levels of Coll2-1 and Coll2-1NO2 were slightly decreased by marathon, indicating that intensive running could reduce cartilage catabolism. The variation of total MPO during the marathon was negatively correlated with the training time per week (r = -0.34 p = 0.009). The active MPO/total MPO ratio was significantly enhanced after the marathon (p < 0.001). The total and active MPO concentrations were increased by 2 to 3-fold in comparison to the pre-marathon values (p < 0.001 for total and active MPO). After the marathon, Coll2-1 and Coll2-1NO2 concentrations were slightly but systematically decreased. The levels of total MPO were higher in female than in male (p < 0.05), but were not affected by the other parameters. The levels of Coll2-1, Coll2-1NO2 and active MPO were not affected by age, body mass index, sex or performance. The subjects were submitted to a questionnaire concerning their physical activity and their life style. Sera were taken at rest right before the departure and within 30 min after the marathon. Coll2-1, Coll2-1NO2, total and active MPO were measured in 98 marathon runners without joint pain and with an average age of 47 years. To determine the influence of marathon on the serum levels of two markers of cartilage degradation, Coll2-1 and its nitrated form, Coll2-1NO2, and of a marker of neutrophils activation, the myeloperoxidase (MPO).

Collagen catabolism